06. MARCH 2019 Experimental Description
→HAIROIDs (date of fixation 30. JUNE 2018) were immersed in 4%PFA (5 Eppis-008) and stored at 4°C until procedure start
• Samples: 008-23; 008-24; 008-32; 008-16; 008-47
→HAROIDs were embedded in cryomatrix
• From the first HAIROID (008-23), BF were taken to recognize the morphology of the HAIROID and to check hair follicle-like structures
→Consecutive 6 µm sections were recollected from the entire HAIROID.
HAIROID ™️ 8-32. SLIDE 12.3: Overview of the entire HAROID. Red arrows are showing a hair follicle-like structure where it is possible to observe a clear dermal papila. Blue arrows show crossections of HAIRFOLLICLES.
HAIROID ™️ 8-32.: Consecutive sections showing 2 different HFs. It is possible to observe in both HFs the dermal papila, hair matrix, outer and inner root sheath.
Our project leader wrote in his report:
„In vivo vs. in vitro:
Stemness markers NESTIN, SOX-2, p63 are present in vivo and in vitro
In agreement with the literature these stem cells are Keratin 14 and 15 negative
Expression needs to be further verified via gene expression analysis
Assay design for optimization of stemness promoting conditions ongoing
Next Step extraction of cells of higher potency (LHX2/SOX9+)
Stable in vitro cultures of potential DP or better HFSC over at least 12 passages
Stability of proliferation rate will be further assessed, but can be considered as stable
Cryopreservation of HFSC is working efficiently
Reprograming from this population will be tested Q1 2018
Non-invasive extraction from hair will be tested Q1 2018“